|Product Name:||Brinster’s BMOC-3 Medium||Brand:||HUACHENYANG|
|Place Of Origin:||China||Capacity:||500ml|
|Color:||Light Red||Storage:||Avoid Light|
500ml Chemically Defined Medium,
FDA Chemically Defined Medium,
500ml Cell Culture Medium
500ml Chemically Defined Medium Without Any Amino Acids Or Vitamins
Brinster’s BMOC-3 Medium is a Chemically defined medium without any amino acids or vitamins
Brinster's BMOC-3 medium is a simple chemically defined medium designed by Ralph Brinster. Brinster's BMOC-3 medium does not contain any amino acids or vitamins but contains BSA, which can support the maturation of oocytes and mammalian Early development of the embryo.
Initially used for the in vitro culture of mouse oocytes, it can also be used for the culture of other lactating embryos during early development.
It is an essential nutrient element in cell culture, but it is unstable in the solution and will spontaneously degrade. The medium without L-glutamine can adjust the amount of L-glutamine arbitrarily according to the research needs. Adding fresh L-glutamine or its substitutes is more conducive to the growth of cells.
The concentration of glucose can be adjusted at will according to the needs of the research, which is convenient and quick. High-glucose medium is widely used in fast-growing, low-adhesion cells, myeloma cells of hybridomas, cloned cells, transformed cells by DNA transfection, various primary virus host cells, single cell culture, and vaccine production. Production, such as the use of CHO cells to express EPO and produce hepatitis B vaccine. A low glucose medium is more suitable for the culture of slower metabolism, dependent adherent cells.
It is used as an indicator of the pH value in the culture medium to continuously monitor the pH of the culture medium. At low pH values, phenol red makes the culture medium yellow, while at higher pH values it makes the culture medium purple. PH When the value is 7.2-7.4, it is red, which is most suitable for cell culture. However, phenol red also has some disadvantages. Studies have shown that phenol red can mimic the effects of steroid hormones (especially estrogen), so when using estrogen-sensitive cells (such as breast tissue), it is best to use phenol red-free Medium. Phenol red interferes with detection during flow cytometry analysis. In addition, the presence of phenol red in some formulations of serum-free media interferes with sodium-potassium balance.
This product has been sterilized by filtration. Care should be taken during an aseptic operation to avoid contamination.
Do not freeze or thaw the product to keep the product in optimal condition.
This product is for scientific research or further research use only, not for diagnosis or treatment.
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