Ham'S F12k Medium Kaighn's Improved Version With Nutrient Mixture

Ham'S F12k Medium Kaighn's Improved Version With Nutrient Mixture

Ham’s F-12K Medium Kaighn’s Modified Ham’s F-12K Modified Medium with Ham’s F-12 Nutrient Mixture

product description:

Ham's F-12K medium is Kaighn's improved version of Ham's F-12 nutrient mixture. On the basis of Ham's F-12, the concentration of amino acids and pyruvate is increased, the amount of glucose is reduced, and the composition and content of salt are modified ( Konigsbergs).

Product Usage:

Ham’s F-12K Medium was originally designed for the growth of primary human hepatocytes as well as differentiated rat and chicken cells.

For a wide range of cell culture applications, we offer Ham’s F-12K Modified Medium with various components.

Product ingredients:

L-glutamine (Glutamine)

It is an essential nutrient element in cell culture, but it is unstable in the solution and will spontaneously degrade. The medium without L-glutamine can adjust the amount of L-glutamine arbitrarily according to the research needs. Adding fresh L-glutamine or its substitutes is more conducive to the growth of cells.

glucose

The concentration of glucose can be adjusted at will according to the needs of the research, which is convenient and quick. High-glucose medium is widely used in fast-growing, low-adhesion cells, myeloma cells of hybridomas, cloned cells, transformed cells by DNA transfection, various primary virus host cells, single cell culture, and vaccine production. Production, such as the use of CHO cells to express EPO and produce hepatitis B vaccine. A low glucose medium is more suitable for the culture of slower metabolism, dependent adherent cells.

Phenol red

It is used as an indicator of the pH value in the culture medium to continuously monitor the pH of the culture medium. At low pH values, phenol red makes the culture medium yellow, while at higher pH values it makes the culture medium purple. PH When the value is 7.2-7.4, it is red, which is most suitable for cell culture. However, phenol red also has some disadvantages. Studies have shown that phenol red can mimic the effects of steroid hormones (especially estrogen), so when using estrogen-sensitive cells (such as breast tissue), it is best to use phenol red-free Medium. Phenol red interferes with detection during flow cytometry analysis. In addition, the presence of phenol red in some formulations of serum-free media interferes with sodium-potassium balance.

Precautions:

This product has been sterilized by filtration. Care should be taken during an aseptic operation to avoid contamination.

Do not freeze or thaw the product to keep the product in optimal condition.

This product is for scientific research or further research use only, not for diagnosis or treatment.