Serum Free CMRL Chemically Defined Medium With Horse Or Calf Serum

Serum Free CMRL Chemically Defined Medium With Horse Or Calf Serum

CMRL medium for serum-free culture of mammalian cell lines supplemented with horse serum or calf serum

product description:

CMRL medium was designed by Connaught Medical Research Laboratories

Product Usage:

Originally used for serum-free culture of L cells (Earle). After adding horse serum or calf serum, the CMRL medium is especially suitable for the clone culture of monkey kidney cells and the culture of other mammalian cell lines.

This product contains amino acids, vitamins, inorganic salts, and other ingredients required for various types of cell culture, but does not contain proteins, lipids, or any growth factors, so this product should be used with serum or serum-free supplements.

Product ingredients:

NEAA (non-essential amino acids)

On the basis of MEM medium, add L-alanine, L-glutamic acid, L-asparagine, L-aspartic acid, L-proline, L-serine and Glycine contains 7 kinds of NEAA, which can reduce the side effects of non-essential amino acids produced by cells themselves during cell culture, and effectively promote cell proliferation and metabolism.

Phenol red

It is used as an indicator of the pH value in the culture medium to continuously monitor the pH of the culture medium. At low pH values, phenol red makes the culture medium yellow, while at higher pH values it makes the culture medium purple. PH When the value is 7.2-7.4, it is red, which is most suitable for cell culture. However, phenol red also has some disadvantages. Studies have shown that phenol red can mimic the effects of steroid hormones (especially estrogen), so when using estrogen-sensitive cells (such as breast tissue), it is best to use phenol red-free Medium. Phenol red interferes with detection during flow cytometry analysis. In addition, the presence of phenol red in some formulations of serum-free media interferes with sodium-potassium balance.

Penicillin-streptomycin mixed solution is usually also called "double antibody", which is the most commonly used antibiotic to prevent microbial contamination in vitro culture. Among them, penicillin can interfere with the synthesis of bacterial cell walls and is particularly effective against Gram-positive bacteria; while streptomycin Mycin can bind to the 30S subunit of bacterial ribosomes and inhibit the synthesis of bacterial proteins. It is effective against both Gram-negative bacteria and Gram-positive bacteria but is particularly effective against Gram-negative bacteria. The combination of penicillin and streptomycin can prevent most bacterial contamination.

Five advantages of iclean cell culture medium:

1. Complete products, 280 kinds of basic culture media

2. More than 200 kinds of special media with missing components or additional components are available in stock

3. The medium can be customized according to the needs, and the composition/concentration/ph value can be customized, etc.

4. The performance of the product is stable, and it is produced by an automatic filling line, and the difference between product batches is small

5. Strict quality control system, sterility testing, mycoplasma, endotoxin testing, cell growth experiment, light inspection, etc.