500ml DMEM Nutrient Mix F12 Ham'S F 12 Medium For Serum Free Culture

Cell Culture Medium

Ham’s F-12 Nutrient Mixture Serum-Free Ham’s F-12 Cell Culture Medium Base Culture Solution

product description:

Ham's F-12 Nutrient Mixture (Ham's F-12 Nutrient Mixture) was designed by Ham in 1969 based on Ham's F-10 Nutrient Mixture, which was originally used for the serum-free culture of CHO cells.

Product Usage:

Ham's F-12 is often used as the basal medium for serum-free culture. It is especially suitable for the single-cell culture and cloning culture at low serum levels. After adding a serum, it is also widely used in the culture of cancer cells and primary cells, such as rats. Hepatocytes, rat prostate epithelial cells, chondrocytes, rat myoblasts, chicken embryo cells, etc. In addition, after mixing equal volumes of Ham’s F-12 and DMEM, the resulting DMEM/F12 medium is more nutritious and more widely used.

For a wide range of cell culture applications, we offer Ham’s F-12 Modified Medium in a variety of components.

Product ingredients:

L-glutamine (Glutamine)
It is an essential nutrient element in cell culture, but it is unstable in the solution and will spontaneously degrade. The medium without L-glutamine can adjust the amount of L-glutamine arbitrarily according to the research needs. Adding fresh L-glutamine or its substitutes is more conducive to the growth of cells.

Phenol red
It is used as an indicator of the pH value in the culture medium to continuously monitor the pH of the culture medium. At low pH values, phenol red makes the culture medium yellow, while at higher pH values it makes the culture medium purple. PH When the value is 7.2-7.4, it is red, which is most suitable for cell culture. However, phenol red also has some disadvantages. Studies have shown that phenol red can mimic the effects of steroid hormones (especially estrogen), so when using estrogen-sensitive cells (such as breast tissue), it is best to use phenol red-free Medium. Phenol red interferes with detection during flow cytometry analysis. In addition, the presence of phenol red in some formulations of serum-free media interferes with sodium-potassium balance.

Precautions:

This product is sterilized by filtration, and aseptic operation should be paid attention to when using it to avoid contamination;

In order to maintain the best use effect of this product, do not freeze or thaw;

This product is only used for scientific research or further research, not for diagnosis and treatment.

Five advantages of iclean cell culture medium:

1. Complete products, 280 kinds of basic culture media

2. More than 200 kinds of special media with missing components or additional components are available in stock

3. The medium can be customized according to the needs, and the composition/concentration/ph value can be customized, etc.

4. The performance of the product is stable, and it is produced by an automatic filling line, and the difference between product batches is small

5. Strict quality control system, sterility testing, mycoplasma, endotoxin testing, cell growth experiment, light inspection, etc.