Liquid DMEM F12 Cell Culture Medium For Developing Serum Free Medium

Cell Culture Medium

Cell culture medium DMEM/F12 medium Mammalian cell culture medium DMEM cell culture medium

product description:

DMEM/F12 medium is based on DMEM medium, adding richer nutrients in F12 medium, containing a variety of trace elements,

Product Usage:

Widely used in the culture of various mammalian cells. At the same time, DMEM/F12 medium is often used as the basis for developing a serum-free medium, and it is also suitable for the culture of mammalian cells and clone density culture under low serum content. A glucose-free medium can adjust the concentration of glucose at will according to research needs, which is convenient and quick.

For a wide range of cell culture applications, we offer modified DMEM/F12 media with various components.

Product ingredients:

L-glutamine (Glutamine)

It is an essential nutrient element in cell culture, but it is unstable in the solution and will spontaneously degrade. The medium without L-glutamine can adjust the amount of L-glutamine arbitrarily according to the research needs. Adding fresh L-glutamine or its substitutes is more conducive to the growth of cells.

HEPES

It is an excellent biological buffer and has no toxic effect on cells. The medium added with HEPES can maintain a constant pH range for a long time, which can effectively prevent the adverse effects of large pH fluctuations in the culture medium on cell growth. Can be used in CO2-free incubators.

Phenol red

It is used as an indicator of the pH value in the culture medium to continuously monitor the pH of the culture medium. At low pH values, phenol red makes the culture medium yellow, while at higher pH values it makes the culture medium purple. PH When the value is 7.2-7.4, it is red, which is most suitable for cell culture. However, phenol red also has some disadvantages. Studies have shown that phenol red can mimic the effects of steroid hormones (especially estrogen), so when using estrogen-sensitive cells (such as breast tissue), it is best to use phenol red-free Medium. Phenol red interferes with detection during flow cytometry analysis. In addition, the presence of phenol red in some formulations of serum-free media interferes with sodium-potassium balance.

Precautions:

This product is sterilized by filtration, and aseptic operation should be paid attention to when using it to avoid contamination;

In order to maintain the best use effect of this product, do not freeze or thaw;

This product is only used for scientific research or further research, not for diagnosis and treatment.